You can continue to use the PC as usual while it is backing up; changes made after starting the backup are ignored, so you cannot make a mess of things. A snapshot of the drive is created before running the backup so Acronis knows what has to be backed-up.
Gigabyte Aorus GA-AX370-Gaming 5 M/B; AMD Ryzen 1700X; 16gig RAM; 2 x 500 gig Samsung 970 EVO PCIe NVMe, 1 x 250 gig Samsung 960 EVO PCIe NVMe drives + other drives (Windows 10 Pro 64)
Gigabyte Aorus B360 Gaming 3M/B, Intel i5 9400; 16gig RAM; 1 x 500 gig Samsung EVO Plus PCIe NVMe + 1 x 250 gig Samsung 960 EVO PCIe NVMe + other drives (Windows 10 Enterprise 64)
Gigabyte Aorus H370 Gaming 3M/B, Intel i5 9400; 16gig RAM; 1 x 500 gig Samsung EVO Plus PCIe NVMe + 3 x Kingston HyerX Fury 240gig RAID 5 + other drives (Windows 10 Enterprise 64)
Synology DS414 NAS 4 x 4TB WD Red HDD